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Abstract

Moringa seed (Moringa oleifera L.) and Chinese betel leaf (Peperomia pellucida L.) extracts obtained using ethanol contain phenolic compounds that function as natural antioxidant compounds. research aimed to quantify the overall phenolic content and assess the antioxidant activity of both extracts using Folin-Ciocalteu and DPPH techniques. The extraction process was performed using the maceration technique with 96% ethanol as the solvent, after which analysis It was performed to determine the overall concentration of phenolic compounds and IC50 a measurement that served as an index of antioxidant power. The findings showed that the extract from Chinese betel leaves had a higher a total phenolic content of 91.02 mg gallic acid equivalents (GAE) was recorded. compared to Moringa seeds which had 54.90 mg GAE/g±0.47. Antioxidant activity testing revealed that the IC50 value of Chinese betel leaf extract reached 3,349 ppm, while moringa seed extract showed an IC50 value of 4,701 ppm. For comparison, vitamin C had a lower IC50 of 1,988 ppm. With IC50 values below 50 ppm, both extracts are categorized as very strong antioxidants.

Keywords

Moringa Seed Chinese Betel Leaf Extract Total Phenolic Antioxidant

Article Details

References

  1. Abdul, A. (2020). Uji Aktivitas Antioksidan Ekstrak Etanol Daun Adas (Foeniculum Vulgare Mill) Dengan Metode DPPH Dan FRAP. Pharmed: Journal of Pharmaceutical Science and Medical Research, 3(2), 43–54.
  2. Adawiah, A., Sukandar, D., & Muawanah, A. (2015). Aktivitas antioksidan dan kandungan komponen bioaktif sari buah namnam. Jurnal Kimia VALENSI, 1(2), 130–136.
  3. Ahwan, A., Suwarni, A., Ariastuti, R., Hafidz, R., & Enjelina, S. M. (2024). Effect Of Total Phenolic And Total Flavonoid Levels On The Antioxidant Power Of Water Extract, Ethanol And Chloroform Of Green Tea Leaves (Camellia sinensis L). Medical Sains: Jurnal Ilmiah Kefarmasian, 9(1), 17–28.
  4. Andriani, D., & Murtisiwi, L. (2018). Penetapan kadar fenolik total ekstrak etanol bunga telang (Clitoria ternatea L.) dengan Spektrofotometri UV Vis. Cendekia Journal of Pharmacy, 2(1), 32–38.
  5. Berniyanti, T. (2020). Biomarker Toksisitas: Paparan Logam Tingkat Molekuler. Airlangga University Press.
  6. Desty, A. S., & Adiprahara, A. M. (2022). Determination of Total Phenolic, Total Flavonoid and Antioxidant Activity of Batak Onion Extract (Allium chinense G. Don). Indonesian Journal of Chemical Science, 11(3). http://journal.unnes.ac.id/sju/index.php/ijcs
  7. Dising, J., & Pasau, P. (2022). Identifikasi senyawa metabolit sekunder dalam ekstrak biji kelor (Moringa Oleifera L) yang berpotensi sebagai antioksidan. Partner, 27(1), 1700–1709.
  8. Elsayed Azab, A., A Adwas, Almokhtar, Ibrahim Elsayed, A. S., A Adwas, A., Ibrahim Elsayed, Ata Sedik, & Quwaydir, F. A. (2019). Oxidative stress and antioxidant mechanisms in human body. Journal of Applied Biotechnology & Bioengineering, 6(1), 43–47. https://doi.org/10.15406/jabb.2019.06.00173
  9. Harborne, J. B. (1987). Chemical signals in the ecosystem. Annals of Botany, 39–57.
  10. Hasanuddin, A. R. P. (2023). Analisis Kadar Antioksidan Pada Ekstrak Daun Binahong Hijau (Anredera cordifolia (Ten.) Steenis). Bioma: Jurnal Biologi Makassar, 8(2), 66–74.
  11. Hidayah, L. A., & Anggarani, M. A. (2022). Determination of total phenolic, total flavonoid, and antioxidant activity of India Onion Extract. Indonesian Journal of Chemical Science, 11(2), 123–135.
  12. Kumontoy, G. D. (2023). Pemanfaatan tanaman herbal sebagai obat tradisional untuk kesehatan masyarakat di Desa Guaan Kecamatan Mooat Kabupaten Bolaang Mongondow Timur. HOLISTIK, Journal of Social and Culture.
  13. Masykuroh, A., & Hidayati, S. (2023). an Antioxidant Activities of Urena lobata L. Ethanolic Extract by DPPH Method: Uji Aktivitas Antioksidan Ekstrak Etanol Bunga Pulutan (Urena lobata L) Menggunakan Metode DPPH. Jurnal Komunitas Farmasi Nasional, 3(1).
  14. Meianti, D. S. D., & Manalu, R. T. (2022). Potensi Antimikroba Ekstrak Etanol Daun Gatal (Urticastrum decumanum (Roxb.) Kuntze) Terhadap Pertumbuhan Staphylococcus aureus dan Candida albicans. Sainstech Farma: Jurnal Ilmu Kefarmasian, 15(2), 93–102.
  15. Mulyani, T., Ariyani, H., Rahimah, R., & Rahmi, S. (2018). Formulasi dan aktivitas antioksidan lotion ekstrak daun suruhan (Peperomia pellucida L.). JCPS (Journal of Current Pharmaceutical Sciences), 2(1), 111–117.
  16. Phaniendra, A., Jestadi, D. B., & Periyasamy, L. (2015, January 1). Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases. Indian Journal of Clinical Biochemistry, 30(1), 11–26. https://doi.org/10.1007/s12291-014-0446-0
  17. Purwanto, D., Bahri, S., & Ridhay, A. (2017). Uji aktivitas antioksidan ekstrak buah purnajiwa (Kopsia arborea Blume.) dengan berbagai pelarut. Kovalen: Jurnal Riset Kimia, 3(1), 24–32.
  18. Ramadhan, N. U., & Ariastuti, R. (2024a). Uji Kandungan Fenolik Total Ekstrak Etanol Daun dan Kulit Batang Kelor (Moringa Oleifera). FASKES: Jurnal Farmasi, Kesehatan, Dan Sains, 2(1), 136–143.
  19. Ramadhan, N. U., & Ariastuti, R. (2024b). Uji Kandungan Fenolik Total Ekstrak Etanol Daun dan Kulit Batang Kelor (Moringa Oleifera). FASKES: Jurnal Farmasi, Kesehatan, Dan Sains, 2(1), 136–143.
  20. Riyanti, S., Jariya, A., & Syahputri, E. Q. (2024). Mini Review Tinjauan Farmakognosi dan Pemanfaatan Tanaman Kelor (Moringa oleifera L.) Sebagai Antidiabetes. Majalah Farmasetika, 9(7), 1–10.
  21. Suhaling, S. (2010). Uji aktivitas antioksidan ekstrak metanol kacang merah (Phaseolus vulgaris L.) dengan metode DPPH. Universitas Islam Negeri Alauddin Makassar.
  22. Sukma, M. (2022). Total Fenolik dan Aktivitas Antioksidan Seduhan Kulit Batang Soni (Dillenia serrata Thunb). Sains: Jurnal Kimia Dan Pendidikan Kimia, 11(1), 27–34.
  23. Susiloningrum, D., & Sari, D. E. M. (2021). Uji Aktivitas Antioksidan dan Penetapan Kadar Flavonoid Total Ekstrak Temu Mangga (Curcuma mangga Valeton & Zijp) dengan Variasi Konsentrasi Pelarut. Cendekia Journal of Pharmacy, 5(2), 117–127.
  24. Yuslianti, E. R. (2018). Pengantar radikal bebas dan antioksidan. Deepublish.
  25. Yusniawati, W. (2024). Uji Kadar Fenolik Total dan Aktivitas Antioksidan Ekstrak Etanol Kunyit Hitam (Curcuma caesia Roxb.) Asal Kabupaten Bone dengan Metode DPPH. Jurnal Novem Medika Farmasi, 3(1).