Article Sidebar

Submitted
Oct 1, 2018
Published
May 15, 2019
Download
pdf
Statistic
Read Counter : 1954 Download : 3082

Downloads

Download data is not yet available.

Main Article Content

Abstract

The rate of infection, especially in Indonesia is still high. Treatment of infections by using antibiotics is constrained by cases of resistance to antibiotics so that it is necessary to explore microorganisms that can produce antibiotics. Antibiotics produced by KP13 bacterial isolates have the potential as antibiotics against Escherichia coli. The purpose of this study was to determine the TLC-Bioautography profile and Densitometry analysis of the most active fraction of KP13 bacterial isolates which have the potential as antibiotics against Escherichia coli bacteria. TLC using the mobile phase Chloroform: Ethylacetate: Methanol (4: 1: 0.5). Active compounds were analyzed using GC-MS. The results showed active spots during TLC-Biaoautography with a Rf value of 0.78. Compounds that are thought to be responsible for antibiotic activity detected by GC-MS at a Rf value of 0.78 have similarities with the compounds of cycloheptatriene and tetrahydrophyran..

Keywords

Densitometry, Escherichia coli, KP13 Isolate, TLC-Bioautography

Article Details

References

  1. B. C. P. Indrayudha dan M Kuswandi, “Chudlori, B., Kuswandi, M., Indrayudha, P., 2012, Pola Kuman dan Resistensinya Terhadap Antibiotika dari Spesimen PUS di RSUD Dr. Moewardi Tahun 2012, Pharmacon,” Pharmaceutical Journal of Indonesia, vol. 13, no. 2, hlm. 1411–4283, 2012.
  2. T. Rahayu, “MULTIRESISTEN BACTERIA,” hlm. 11, 2007.
  3. H. Rante dan Y. B. Murti, “Purifikasi dan karakterisasi senyawa anti-bakteri dari actinomycetes asosiasi spons terhadap bakteri patogen resisten,” hlm. 8, 2010.
  4. I. Narwanti dan N. Sulistyani, “TLC-Bioautography Profile of Ethyl Acetate Extract of 5 Bacteria Isolated from Ficus carica L Rhizosphere,” Int. J. Public Health Sci. IJPHS, vol. 4, no. 2, hlm. 81, Jun 2015.
  5. A. Syarifuddin dan N. Sulistyani, “Activity of Antibiotic Bacterial Isolate Kp13 and Cell Leakage Analysis of Escherichia coli Bacteria,” J. Ilmu Kefarmasian Indones., vol. 16, no. 2, hlm. 137–144, Okt 2018.
  6. J. Widada dan W. Asmara, “Antifungal Production of a Strain of Actinomycetes spp Isolated from the Rhizosphere of Cajuput Plant: Selection and Detection of Exhibiting Activity Against Tested Fungi,” hlm. 10.
  7. X. Wang, L. Huang, Z. Kang, H. Buchenauer, dan X. Gao, “Optimization of the Fermentation Process of Actinomycete Strain Hhs.015 T,” J. Biomed. Biotechnol., vol. 2010, hlm. 1–10, 2010.
  8. Department of Microbiology, Hindushtan College of Arts and Science, Coimbatore-28, India dan H. N, “PURIFICATION OF SECONDARY METABOLITES FROM SOIL ACTINOMYCETES,” Int. J. Microbiol. Res., vol. 3, no. 3, hlm. 148–156, Des 2011.
  9. Mulyadi dan N. Sulistyani, “AKTIVITAS CAIRAN KULTUR 12 ISOLAT ACTINOMYCETES TERHADAP BAKTERI RESISTEN,” J. Kesehat. Masy. J. Public Health, vol. 7, no. 2, Des 2013.
  10. H. Marisa, “Isolasi Senyawa Antibakteri Dari Daun Jengkol (Pithecolobium lobatum Benth) dan Penentuan Nilai KHM-nya,” hlm. 4.
  11. E. Kusumaningtyas dan E. Astuti, “Sensitivitas Metode Bioautografi Kontak dan Agar Overlay dalam Penentuan Senyawa Antikapang,” vol. 6, hlm. 6, 2008.
  12. P. Moncheva, S. Tishkov, N. Dimitrova, V. Chipeva, S. Antonova-Nikolova, dan N. Bogatzevska, “CHARACTERISTICS OF SOIL ACTINOMYCETES FROM ANTARCTICA,” hlm. 12.
  13. L. P. Y. G. A. Aleksandrova dan T. A. Akent’eva, “Synthesis and Antimicrobial Activity of Amines and Imines with a Cycloheptatriene Fragment,” Pharmaceutical Chemistry Journal, vol. 46, no. 12, 2013.