Article Sidebar

Submitted
May 5, 2020
Published
May 1, 2021
Download
Pdf
Statistic
Read Counter : 750 Download : 1629

Downloads

Download data is not yet available.

Main Article Content

Abstract

The search for new Antibiotics is needed in the hospital because of the multi-resistance Antibiotics that result in an increase in deaths due to Antibiotic abuse in dealing with infectious diseases, so it is necessary to explore to get potential new antibiotics. Actinomycetes are microorganisms that can produce is antibiotics. Actinomycetes isolate (isolate TE 235) has been isolated from rizosphere soil of sugarcane root crops. This study aimed to determine the optimal time of TE 235 isolates in producing secondary metabolites in the form of antibiotics that can test bacteria Escherichia coli and Staphylococcus aureus and the growth profile of TE 235 isolates were reviewed based on the production of cell biomass. The activity test method used in this study is a suitable test method. The results showed that the liquid culture of Actinomycetes isolates (isolate TE 235) had an optimal time to produce secondary metabolites (Antibiotics) against Staphylococcus aureus bacteria after incubation for ten days with inhibition zone diameter of 6.67 ± 0.94 mm, whereas against Escherichia coli bacteria on the seventh day with a diameter of inhibitory zone of 10.00 ± 0.82 mm. The results of research on the growth of Actinomycetes bacteria isolate TE 235 based on the weight of cell biomass that underwent the stationary phase on the 9th day. The incubation time of the culture of Isolate TE 235 to obtain optimal approval of antibiotics after ten days of incubation in terms of antibiotic activity on Staphylococcus aureus and Escherichia coli and the growth phase of TE235 isolates as the stationary phase.

Keywords

Antibiotic Optimization Secondary Metabolites TE 234 isolates

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

References

  1. Aini, N. N., & Sulistyani, N. (2019). Isolation of Actinomycetes from Sugarcane (Saccharum officinarum) Rhizosphere and the Ability to Produce Antibiotic. Proceedings of the 2019 Ahmad Dahlan International
  2. Conference Series on Pharmacy and Health Science (ADICS-PHS 2019). Proceedings of the 2019 Ahmad Dahlan International Conference Series on Pharmacy and Health Science (ADICS-PHS 2019), Yogyakarta, Indonesia. https://doi.org/10.2991/adics-phs-19.2019.3
  3. Indrawati, I., & Rizki, A. F. M. (2017). Potensi Ekstrak Buah Buni (Antidesma bunius L) Sebagai Antibakteri Dengan Bakteri Uji Salmonella thypimurium Dan Bacillus cereus. Jurnal Biodjati, 2(2), 138–148.
  4. Ismail, S. A., El-Modather, A., Ewais, E. A., & Refaat, B. M. (2017). Antimicrobial Activity of Isolated Actinomycetes and Optimization of Bioactive Metabolites Production. Inventi Rapid: Pharm Biotech & Microbio, 2017(3), 9.
  5. Katili, A. S. (2017). Short Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia. Biodiversitas, Journal of Biological Diversity, 18(2), 826–833. https://doi.org/10.13057/biodiv/d180256
  6. Lani, M. N. (2014). Antimicrobial Activity of Cell Free Supernatant of Lactic Acid Bacteria Isolated from Fermented Durian Flesh against Multiple Antibiotic Resistance’s Salmonella Associated with Food Poisoning Cases in Malaysia. IOSR Journal of Pharmacy and Biological Sciences, 9(6), 60–65. https://doi.org/10.9790/3008-09646065
  7. Mitrovic, I., Grahovac, J., Dodic, J., Grahovac, M., Dodic, S., Vucurovic, D., & Vlajkov, V. (2017). Effect of agitation rate on the production of antifungal metabolites by Streptomyces hygroscopicus in a lab-scale bioreactor. Acta Periodica Technologica, 48, 231–244. https://doi.org/10.2298/APT1748231M
  8. Mohamed, H., Miloud, B., Zohra, F., García-Arenzana, María, J., Veloso, A., & Rodríguez-Couto, S. (2017). Isolation and Characterization of Actinobacteria from Algerian Sahara Soils with Antimicrobial Activities. Int J Mol Cell Med Spring, 6(2), 110–120. https://doi.org/10.22088/acadpub.BUMS.6.2.5
  9. Mulyadi, ., & Sulistyani, N. (2013). Aktivitas Cairan Kultur 12 Isolat Actinomycetes Terhadap Bakteri Resisten. Jurnal Kesehatan Masyarakat (Journal of Public Health), 7(2), 89–96. https://doi.org/10.12928/kesmas.v7i2.1043
  10. Oskay, M. (2011). Effects of some Environmental Conditions on Biomass and Antimicrobial Metabolite Production by Streptomyces Sp. Internasional Journal of Agriculture & Biology, January 2011, 317–324.
  11. Peddi, P., & Donthireddy, S. R. R. (2018). Optimization of process parameters for the production of bioactive compound by marine actinomycetes Streptomyces enissocaesilis PVNRHB2 under submerged fermentation. European Journal of Biotechnology and Bioscience, 6(5), 80–85.
  12. Ramadhan, H. (2019). Isolasi Actinomycetes Penghasil Antibiotik Terhadap Escherichia coli Dan Staphylococcus aureus Dari Tanah Sawah. Jurnal stikes borneo lestari banjar baru, 50–64.
  13. Ramadhani, M. A., & Sulistyan, N. (2018). Uji Aktivitas Isolat Actinomycetes (Kode Gst, Kp, Kp11, Kp16, T24, Dan T37) Terhadap Staphylococcus Aureus Atcc 25923 Dan Escherichia Coli Atcc 25922. 01(02), 29–37. https://doi.org/NOISSN: 2615-6903
  14. Satria, H. (2011). Kinetika Fermentasi Produksi Selulase Dari Isolat Actinomycetes ACP-7 Pada Media Pada Jerami Padi. Jurnal Kimia Kemasan, 33(2), 152–159.
  15. Syarifuddin, A., Kamal, S., Yuliastuti, F., Pradani, M. P. K., & Septianingrum, N. M. A. N. (2019). Ekstraksi Dan Identifikasi Metabolit Sekunder Dari Isolat AL 6 Serta Potensinya Sebagai Antibakteri Terhadap Escherichia coli. Bioteknologi & Biosains Indonesia, 6(2), 210–218. https://doi.org/ISSN 2548 – 611X
  16. Syarifuddin, A., Sulistyani, N., & Kintoko, K. (2018). Activity of Antibiotic Bacterial Isolate Kp13 and Cell Leakage Analysis of Escherichia coli Bacteria. Jurnal Ilmu Kefarmasian Indonesia, 16(2), 137. https://doi.org/10.35814/jifi.v16i2.529
  17. Tandon, C. (2015). Andrographis paniculata Nees ( Kalmegh ): A Review on Its Antibacterial Activities and Phytocompounds. European Journal of Medicinal Plants, 8(1), 1–10. https://doi.org/10.9734/EJMP/2015/16280
  18. Warsi, W., & Sulistyani, N. (2018). The Optimization of Secondary Metabolite Production Time and Screening Antibacterial Activity of Actinomycetes Isolate from Tin Plant Rizosfer (Ficus carica). Jurnal Teknologi Laboratorium, 7(1), 15. https://doi.org/10.29238/teknolabjournal.v7i1.120
  19. Wulandari, W., & Rahayu, T. (2015). Aktivitas Antibakteri Isolat Actinomycetes Dari Sampel Pasir Gunung Merapi Dengan Lama Fermentasi Yang Berbeda Terhadap Bakteri Escherichia coli Multiresisten Antibiotik. Bioeksperimen, 1(2), 53–59. https://doi.org/ISSN 2460-1365
  20. Yuliastuti, F., Hapsari, W. S., Syarifuddin, A., & Pradani, M. P. K. (2020). Fractionation antibiotic isolate: Trial protocol for antibiotic compound from Barleria prionitis L. extract. Technology Reports of Kansai University, 62(03), 6.