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Mar 28, 2021
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Nov 1, 2021
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Abstract

Fennel leaf (Foeniculum vulgare Mill) is an herbal plant that is used for
treatment. Flavonoid compounds and essential oils contained in fennel leaves
have an antibacterial effect. The purpose of this study was to determine the
activity of fennel leaf extract against Pseudomonas aeruginosa bacteria.
Testing of antibacterial activity using a pure experimental method with a
randomized design with a unidirectional pattern using the extraction method,
namely maceration and bacterial activity testing with the diffusion method. The
parameter measured is the diameter of the inhibition formed in the paper disc
area. The data were measured using the Anova statistical approach. Fennel leaf
extract has an inhibitory effect on the growth of Pseudomonas aeruginosa
bacteria, namely at concentrations of 15% (11.04 ± 1.59 mm), 25% (13.68 ±
3.54 mm), 50% (18.14 ± 2.04 mm), 75% (20.53 ± 0.88 mm) and 100% (22.82 ±
0.32 mm) compared with 1% Dimethyl Sulfoxide (DMSO) negative control
(00.00 ± 0.00 mm), while the test with positive control Gentamicin 1% (22.82 ±
0.32 mm) with a strong category (10 - 20 mm), namely at concentrations of
15%, 25% and 50%, very strong category (>20mm), namely at concentrations
of 75% and 100%, the statistical significance value of ANOVA was p<0.05. It
can be concluded that the ethanol extract of fennel leaf (Foenicullum vulgare
Mill) has antibacterial activity against Pseudomonas aeruginosa. The value of
the minimum inhibitory concentration (MIC) of ethanol extract of fennel leaf
(Foenicullum vulgare Mill) against Pseudomonas aeruginosa bacteria was
15% w/v.

Keywords

Antibacterial Pseudomonas aeruginosa Ethanol Extract Fennel Leaves

Article Details

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