Article Sidebar

Submitted
Apr 20, 2021
Published
Apr 28, 2022
Download
Pdf
Statistic
Read Counter : 661 Download : 3584

Downloads

Download data is not yet available.

Main Article Content

Abstract

Flavonoid is the compounds in nature that play a role in increasing body immunity. Flavonoids in green plants can be sourced from fennel leaves. The aims of the study were to determine the total levels of flavonoids in fennel leaves (Foeniculum vulgare) using Visible Spectrophotometry. Maceration extracted samples using ethanol 96% solvent. Qualitative analysis of flavonoids using dilute NaOH, Wilstater-cyanidin method, and AlCl3. Quersetin used as standard solution. Quantitative analysis was carried out by colorimetric method with ALCl3 reagent as a complex compound. Quantitative analysis was continued with Visible Spectrophotometry on operating time at the 28th minute and a wavelength of 430.5 nm. Qualitative analysis indicate that the ethanol extract of fennel leaves was positive flavonoids. The total flavonoid content of fennel leaves ethanol extract was 99.2 mg QE/g extract with a coefficient of variation 1.27%.

Keywords

Fennel Leaves Total Flavonoid Colorimetry Visible Spectrophotometry

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

References

  1. Abdul, A., & Qonitah, F. (2019). Pengaruh Pemberian Ekstrak Etanol Daun Adas terhadap Kadar Hormon Prolaktin pada Tikus Betina Post Partum. Jurnal Farmasetis, 8(2), 39–44.
  2. Asmorowati, H., & Lindawati, N. Y. (2019). Penetapan kadar flavonoid total alpukat ( Persea americana Mill .) dengan metode spektrofotometri. Ilmiah Farmasi, 15(2), 51–63.
  3. Dua, A., Mittal, A., Gupta, S., & Mahajan, R. (2013). Bioreactive Compounds and Antioxidant Properties of Methanolic Extract of Fennel (Foeniculum Vulgare Miller). International Research Journal of Pharmacy, 4(5), 241–245.
  4. Estikawati, I., & Lindawati, N. Y. (2019). Penetapan Kadar Flavonoid Total Buah Oyong (Luffa acutangula (L .) Roxb.) dengan Metode Spektrofotometri UV-Vis. Jurnal Farmasi Sains Dan Praktis, 5(2), 96–105.
  5. Haeria, Tahar, N., & Ramadhani, N. H. (2017). Uji Efektivitas Imunomodulator Ekstrak Etanol Korteks Kayu Jawa (Lannea coromandelica Hout .Merr.) terhadap Aktivitas dan Kapasitas Fagositosis Makrofag pada Mencit (Mus musculus) Jantan. Jf Fik Uinam, 5(4), 294–302.
  6. Kusnadi, & Devi, E. T. (2017). Isolasi dan Identifikasi Senyawa Flavonoid Pada Ekstrak Daun Seledri (Apium graveolens L.) Dengan Metode Refluks. Pancasakti Science Education Journal, 2(9), 56–67.
  7. Lindawati, N. Y., & Ma’ruf, S. H. (2020). Penetapan Kadar Total Flavonoid Ekstrak Etanol Kacang Merah (Phaseolus Vulgaris L.) Secara Spektrofotometri Visibel. Jurnal Ilmiah Manuntung, 6(1), 83.
  8. Marpaung, M. P., & Wahyuni, R. C. (2018). Identifikasi Dan Penetapan Kadar Flavonoid Total Ekstrak Akar Kuning (Fibraurea chloroleuca Miers). Talenta Conference Series: Tropical Medicine (TM), 1(3), 095–098.
  9. Nofita, D., Sari, S. N., & Mardiah, H. (2020). Penentuan Fenolik Total dan Flavonoid Ekstrak Etanol Kulit Batang Matoa (Pometia pinnata J.R& G.Forst) secara Spektrofotometri. Chimica et Natura Acta, 8(1), 36.
  10. Nugraha, T., Kiki, M., & & Kodir, A. R. (2016). Pengujian Aktivitas Antioksidan pada Fraksi Berbeda dan Penentuan Kadar Flavonoid Total Dari Daun Jalantir ( Erigeron sumatrensis Retz .) yang Berasal dari Jawa Barat Indonesia
  11. Antioxidant Activity Test on Different Fraction and Determination of Flavonoid. Prosiding Farmasi, 2(2), 755–762.
  12. Permadi, A., Sutanto, & Wardatun, S. (2015). Perbandingan Metode Ekstraksi Bertingkat Dan Tidak Bertingkat Terhadap Flavonoid Total Herba Ciplukan Secara Kolorimetri. Jurnal Online Mahasiswa Bidang Farmasi, 1(1), 1–10.
  13. Rauf, A., Haeria, & Anas, D. D. (2016). Efek Imunostimulan Fraksi Daun Katuk (Sauropus androgynus L. MERR) Terhadap Aktivitas dan Kapasitas Fagositosis Makrofag pada Mencit Jantan (Mus Muculus). 4(4), 9–15.
  14. Simanjuntak, K. (2012). Peran Antioksidan Flavonoid dalam Meningkatkan Kesehatan. Bina Widya, 23(3), 135–140.
  15. Subositi, A. P. D. (2014). Analisis Ukuran Partikel Bahan Penyusun Ramuan Jamu Dan Volume Air Penyari Terhadap Mutu Ekstrak Yang Dihasilkan. Jurnal Ilmu Farmasi Dan Farmasi Klinik, 111–115.
  16. Wahyulianingsih, W., Handayani, S., & Malik, A. (2016). Penetapan Kadar Flavonoid Total Ekstrak Daun Cengkeh (Syzygium aromaticum (L.) Merr & Perry). Jurnal Fitofarmaka Indonesia, 3(2), 188–193.
  17. Wisudyaningsih, B. (2012). Studi preformulasi: validasi metode spektrofotometri ofloksasin dalam larutan dapar fosfat. Stomatognatic, 9(2), 77–81.
  18. Yuda, I. K. A., Anthara, M. S., & Dharmayudha, A. A. G. O. (2013). Identifikasi Golongan Senyawa Kimia Estrak Etanol Buah Pare (Momordica charantia) dan Pengaruhnya Terhadap Penurunan Kadar Glukosa Darah Tikus Putih Jantan (Rattus novergicus) yang Diinduksi Aloksan. Buletin Veteriner Udayana, 5(2), 87–95.