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Jan 9, 2022
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Jan 25, 2022
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Abstract

Sponges are marine organism that contain various chemical compound with potential pharmacological activities. This study aims to isolate and identify secondary metabolites from ethyl acetate extract of Petrosia sp. and its antioxidant properties. Extraction was performed by maceration using ethyl acetate. Isolation methods were carried out using Thin Layer Chromatography (TLC), Vacuum Liquid Chromatography (VLC), and Radial Chromatography (RC) technique. Isolated compound was identified by 1D-NMR (1H and 13C NMR) and 2D-NMR (HMQC and HMBC). Antioxidant assay was determined by its activity against ABTS radical. Isolated compound identified as an alkaloid namely 6-hydroxy-1,3,7-trimethyl-3,5-dihydro-1-Himidazo [4,5-c] piridine -2,4-dione. Antioxidant activity test showed Petrosia sp. extract was active as antioxidant with IC50 values 27.20 μg/mL against DPPH and 27.53 μg/mL against ABTS, whereas isolated compound was inactive against DPPH and ABTS with IC50 values 242.64 μg/mL. Meanwhile, Vitamin C was very active against DPPH and ABTS with IC50 21.51 μg/mL and 22.21 μg/mL, respectively.

Keywords

Petrosia Antioxidant Isolation Elucidation

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References

  1. Abdel-Lateff, A., Alarif, W. M., Asfour, H. Z., Ayyad, S. E. N., Khedr, A., Badria, F. A., & Al-lihaibi, S. S. (2014). Cytotoxic effects of three new metabolites from Red Sea marine sponge, Petrosia sp. Environmental Toxicology and Pharmacology, 37(3), 928–935. https://doi.org/10.1016/j.etap.2014.03.005
  2. Badarinath, A. V, Rao, K. M., Madhu, C., Chetty, S., Ramkanth, S., Rajan, T. V. S., & Gnanaprakash, K. (2010). A Review on in-vitro antioxidant methods: Comparisions, correlations and considerations. International Journal of PharmTech Research, 2(2), 1276–1285. https://www.sid.ir/en/journal/ViewPaper.aspx?ID=383951
  3. Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28(1), 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5
  4. Clarke, G., Ting, K. N., Wiart, C., & Fry, J. (2013). High Correlation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) Radical Scavenging, Ferric Reducing Activity Potential and Total Phenolics Content Indicates Redundancy in Use of All Three Assays to Screen for Antioxidant Activity of Extracts of Plants from the Malaysian Rainforest. Antioxidants (Basel, Switzerland), 2(1), 1–10. https://doi.org/10.3390/ANTIOX2010001
  5. Dewi, N. W. O. A. C., Puspawati, N. M., Swantara, I. M. D., Asih, I. A. R. A., & Rita, W. S. (2014). Aktivitas Antioksidan Senyawa Flavonoid Ekstrak Etanol Biji Terong Belanda (Solanum betaceum, syn) dalam Menghambar Reaksi Peroksidasi Lemak pada Plasma Darah Tikus Wistar. Cakra Kimia, 2(1), 7–16. https://ojs.unud.ac.id/index.php/cakra/article/view/9002/6781
  6. Fristiohady, A., Malaka, M. H., Safitri, A. R. W., Diha, D., Saripuddin, Julian Purnama, L. O. M., Rahman, S., Yodha, A. W. M., Munasari, D., Sadarun, B., & Sahidin, I. (2021). Anti-Inflammatory Activity of Ethanol Extract of Marine Sponge Petrosia Sp. by Supression the Level of Tumor Necrosis Factor-alpha. Research Journal of Pharmacy and Technology, 14(8), 4435-4439. https://doi.org/10.52711/0974-360X.2021.00770
  7. Haedar, H., Sadarun, B., & Palupi, R. D. (2016). Potensi Keanekaragaman Jenis dan Sebaran Spons di Perairan Pulau Saponda Laut Kabupaten Konawe. Sapa Laut, 1(9), 1–9. http://ojs.uho.ac.id/index.php/jsl
  8. Handayani, D., Handayani, C., & Krisyanella, K. (2012). Isolasi Senyawa Kimia Utama dari Fraksi Aktif Sitotoksik Spon Laut Petrosia sp (MN05). Jurnal Farmasi Higea, 4(1), 24–30.
  9. Handayani, D., Sayuti, N., Dachriyanus, D., & van Soest, R. W. M. (2011). Epidioksi Sterol , Senyawa Antibakteri dari Spon Laut Petrosia nigrans. Jurnal Bahan Alam Indonesia, 7(6), 289–293. https://www.researchgate.net/publication/282946738_Epidioksi_Sterol_Senyawa_Antibakteri_dari_Spon_Laut_Petrosia_nigrans
  10. Hitora, Y., Takada, K., Okada, S., Ise, Y., & Matsunaga, S. (2011). (-)-Duryne and its homologues, cytotoxic acetylenes from a marine sponge Petrosia sp. Journal of Natural Products, 74(5), 1262–1267. https://doi.org/10.1021/NP200271N/SUPPL_FILE/NP200271N_SI_001.PDF
  11. Kurnianda, V., Ramadhan, M. R., Karina, S., Agustina, S., Octavina, C., Ulfah, M., Syahliza, F., Faradilla, S. B., Putra, D. F., Purnawan, S., & Musman, M. (2019). The inhibitor of human bladder epithelial cancer cells from Indonesian marine sponge of Petrosia sp. IOP Conference Series: Earth and Environmental Science, 348(1), 1–5. https://doi.org/10.1088/1755-1315/348/1/012025
  12. Molyneux, P. (2004). The Use Of The Stable Free Radical Diphenylpicryl-hydrazyl (DPPH) For Estimating Anti-oxidant Activity. Songklanakarin Journal of Science and Technology, 26(May), 1–10.
  13. Nhiem, N. X., Quang, N. Van, Minh, C. Van, Hang, D. T. T., Anh, H. L. T., Tai, B. H., Yen, P. H., Hoai, N. T., Thung, D. C., & Van Kiem, P. (2013). Biscembranoids from the marine sponge Petrosia nigricans. Natural Product Communications, 8(9), 1209–1212. https://doi.org/10.1177/1934578x1300800905
  14. Pailee, P., Mahidol, C., Ruchirawat, S., & Prachyawarakorn, V. (2017). Sterols from Thai Marine Sponge Petrosia (Strongylophora) sp. and Their Cytotoxicity. Marine Drugs, 15(3). https://doi.org/10.3390/MD15030054
  15. Rahman, S. R., Sadarun, B., & Rahmadani, R. (2020). Sponge Community Structure in Lalanu Seawaters, Soropia of Southeast Sulawesi. Sapa Laut, 5(3), 183–192.
  16. Sadarun, B., Malaka, M. H., Wahyuni, W., & Sahidin, S. (2017). Toksisitas Akut Senyawa Metabolit Sekunder dari Spons Laut Clathria sp. Phamauho: Jurnal Farmasi, Sains, Dan Kesehatan, 3(1), 6–9. http://ojs.uho.ac.id/index.php/pharmauho/article/view/3445/2599
  17. Sadarun, B., Malaka, M. H., Wahyuni, W., & Sahidin, S. (2018). Senyawa Steroid Spons Xestospongia sp. dari Perairan Sulawesi Tenggara. Pharmauho: Jurnal Farmasi, Sains, Dan Kesehatan, 4(1), 26–29. https://doi.org/10.33772/pharmauho.v4i1.4628
  18. Sahidin, I., Sabandar, C. W., Wahyuni, W., Hamsidi, R., Malaka, M. H., Sadarun, B., & Aslan, L. O. (2018). A-nor steroids from the marine sponge, Clathria species. Malaysian Journal of Analytical Sciences, 22(3), 375–382. https://doi.org/10.17576/mjas-2018-2203-02
  19. Vifta, R., Wilantika, W., & Advistasari, Y. D. (2019). Study in Vitro of Antioxidant Potency and Antidiabetic Activity of Ethyl Acetate Fraction of Parijoto Fruit (Medinilla speciosa B.). Jurnal Tumbuhan Obat Indonesia, 12(2), 93–102. https://doi.org/10.22435/jtoi.v12i2.1160
  20. Yamin, Ruslin, Sartinah, A., Ihsan, S., Kasmawati, H., Suryani, Andriyani, R., Asma, Adjeng, A. N. T., & Arba, M. (2020). Radical scavenging assay and determination flavonoid and phenolic total of extract and fractions of Raghu bark (Dracontomelon dao (blanco) merr). Research Journal of Pharmacy and Technology, 13(5), 2335–2339. https://doi.org/10.5958/0974-360X.2020.00420.5