Article Sidebar

Submitted
Feb 23, 2023
Published
Jan 31, 2024
Download
Pdf
Statistic
Read Counter : 181 Download : 164

Downloads

Download data is not yet available.

Main Article Content

Abstract

Biofilm is a common causative factor for urinary tract infections due to catheter usage with a percentage of infection around 70-80%. The Bajakah tampala (Spatholobus littoralis Hassk) is one of native plants of Kalimantan which contains phenolic compounds, flavonoids and tannins which are proven to accelerate wound healing, have antibacterial activity, and have very high and strong antioxidant activity. This study aims to determine the antibacterial and anti-biofilm activity of bajakah root extract (Spatholobus littoralis Hassk)  on catheter colonies of Staphylococcus aureus, Escherichia coli and Candida albicans and to determine their mechanism of action in vitro. This research is carried out with an in vitro experimental study design using a microplate reader. Extraction was carried out by maceration method using 96% ethanol solvent. The results showed that the ethanol extract of Bajakah tampala (Spatholobus littoralis Hassk) had antibacterial activity against S. aureus with a concentration of 1% w/v of 88.33% ± 0.01 and anti-biofilm activity of 82.21% ± 0.01. E. Coli bacteria had an antibacterial activity of 84.83% and an anti-biofilm activity of 80.11 at a concentration of 1% w/v. C. albicans had an antifungal activity at a concentration of 1% w/v of 82.31% ± 0.01 and anti-biofilm activity of 77.00% ± 0.01. From these results it can be concluded that the ethanol extract of Bajakah tampala (Spatholobus littoralis Hassk) has antibacterial and antifungal activities and the potential as a new anti-biofilm agent against S.aureus, E. coli and C. albicans

Keywords

Bajakah tampala Antibiofilm S.aureus E.Coli C.albicans

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

References

  1. Agustin, B. A., Puspawaty, N., & Rukmana, R. M. (2018). Aktivitas antibakteri kombinasi ekstrak etanolik daun beluntas (pluchaea indica less.) Dan meniran (phyllanthus niruri l.) Terhadap bakteri staphylococcus aureus. Biomedika, 11(2), Article 2. https://doi.org/10.31001/biomedika.v11i2.425
  2. Ayuchecaria, N., Saputera, M. M. A., & Niah, R. (2020). Penetapan kadar fenolik total ekstrak batang bajakah tampala (spatholobus littoralis hassk.) menggunakan spektrofotometri uv-visible. Jurnal Insan Farmasi Indonesia, 3(1), 132–141. https://doi.org/10.36387/jifi.v3i1.478
  3. Ballen, V., Cepas, V., Ratia, C., Gabasa, Y., & Soto, S. M. (2022). Clinical Escherichia coli: From Biofilm Formation to New Antibiofilm Strategies. Microorganisms, 10(6), 1103. https://doi.org/10.3390/microorganisms10061103
  4. Darouiche, R. O. (2001). Device-associated infections: A macroproblem that starts with microadherence. Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America, 33(9), 1567–1572. https://doi.org/10.1086/323130
  5. Febrianti, D. R., Khairina, N., & Alisa, P. N. (2018). Uji aktivitas anti mikroorganisme ekstrak jeringau (acorus calamus l.) terhadap jamur candida albicans dan bakteri staphylococcus aureus. Jurnal Insan Farmasi Indonesia, 1(1), 96–103.
  6. Hamzah, H., Hertiani, T., Pratiwi, S. U. T., Nuryastuti, T., & Murti, Y. B. (2020). The biofilm inhibition and eradication activity of curcumin againts polymicrobial biofilm. BIO Web of Conferences, 28, 04001. https://doi.org/10.1051/bioconf/20202804001
  7. Hamzah, H., Pratiwi, S. U. T., & Hertiani, T. (2018). Efficacy of thymol and eugenol against polymicrobial biofilm. Indonesian Journal of Pharmacy, 29(4), Article 4. https://doi.org/10.14499/indonesianjpharm29iss4pp214
  8. Hamzah, H., Rasdianah, N., Nurwijayanto, A., & Nandini, E. (2021). Aktivitas ekstrak etanol daun calincing terhadap biofilm candida albicans. Jurnal Farmasetis, 10(1), Article 1. https://doi.org/10.32583/farmasetis.v10i1.1319
  9. Hamzah, H., Siregar, K. A. A. K., Nurwijayanto, A., Wahyuningrum, R., & Sari, S. (2021). Effectiveness of oxalis corniculata l. ethanol extract against mono-species of biofilm staphylococcus aureus. Borneo Journal of Pharmacy, 4(3), Article 3. https://doi.org/10.33084/bjop.v4i3.2418
  10. Hasyrul Hamzah, Sylvia Utami Tunjung Pratiwi, Asriullah Jabbar, Aldyba Syaqilla Hafifah, Badarani Abbas Al-Fajri, & Nurhalisah Nurhalisah. (2023). Bioactivity tracing of the ethanol extract of bajakah tampala (spatholobus littoralis hassk.) typical plant of kalimantan island as antibiofilm of staphylococcus aureus | Open Access Macedonian Journal of Medical Sciences. Open Access Macedonian Journal of Medical Sciences, 10(A), 8–14.
  11. Muhlisah, F. (2007). Tanaman obat keluarga toga. Penebar swadaya.
  12. Kurniawan, B. B. (2019). Uji aktivitas antibakteri batang bajakah tampala (spatholobus littoralis hassk.) terhadap bakteri staphylococcus aureus atcc 26923. Pharmacy Jurnal Farmasi Indonesia, 16(2), 178–187.
  13. Mah, T. F., & O’Toole, G. A. (2001). Mechanisms of biofilm resistance to antimicrobial agents. Trends in Microbiology, 9(1), 34–39. https://doi.org/10.1016/s0966-842x(00)01913-2
  14. Marlina & Samad, R. A. (2013). Hubungan pemasangan kateter dengan kejadian infeksi saluran kemih pada pasien di ruang rawat inap penyakit dalam rsudza. Jurnal Keperawatan Medikal Bedah, 1(1), 35–47.
  15. Mochammad Maulidie Alfiannor Saputera, Tio Widia Astuti Marpaung, N. A. (2019). Konsentrasi hambat minimum (khm) kadar ekstrak etanol batang bajakah tampala (spatholobus littoralis hassk) terhadap bakteri escherichia coli melalui metode sumuran. Jurnal Ilmiah Manuntung, 5(2), 167–173.
  16. Mochtar, C. F., Saleh, L. O., Hamzah, H., & Ilyas, N. M. (2022). Potensi bajakah tampala (spatholobus littoralis hassk) sebagai antibakteri dan antijamur terhadap staphylococcus aureus dan candida albicans. Jurnal Mandala Pharmacon Indonesia, 8(2), 177–184. https://doi.org/10.35311/jmpi.v8i2.212
  17. Nicolle, L. E. (2014). Catheter associated urinary tract infections. Antimicrobial Resistance and Infection Control, 3, 23. https://doi.org/10.1186/2047-2994-3-23
  18. Novriyanti, R., Putri, N. E. K., & Rijai, L. (2022). Skrining fitokimia dan uji aktivitas antioksidan ekstrak etanol kulit jeruk nipis (citrus aurantifolia) menggunakan metode dpph: phytochemical screening and antioxidant activity testing ethanol extract of lime skin (citrus aurantifolia) using DPPH method. Proceeding of Mulawarman Pharmaceuticals Conferences, 15, 165–170. https://doi.org/10.25026/mpc.v15i1.637
  19. Nurdin, E., Nurdin, G. M., & Noviyanti, R. (2020). Hubungan durasi pemakaian kateter terhadap infeksi staphylococcus aureus pada pasien infeksi saluran kemih rawat inap. Jurnal Celebes Biodiversitas, 3(2), 25–29.
  20. Pelling, H., Nzakizwanayo, J., Milo, S., Denham, E. L., MacFarlane, W. M., Bock, L. J., Sutton, J. M., & Jones, B. V. (2019). Bacterial biofilm formation on indwelling urethral catheters. Letters in Applied Microbiology, 68(4), 277–293. https://doi.org/10.1111/lam.13144
  21. Saputera, M. M. A., & Ayuchecaria, N. (2018). Uji efektivitas ekstrak etanolik batang bajakah tampala (spatholobus littoralis hassk.) terhadap waktu penyembuhan luka. Jurnal Ilmiah Ibnu Sina, 3(2), 318–327. https://doi.org/10.36387/jiis.v3i2.185
  22. Saputera, M. M. A., Marpaung, T. W. A., & Ayuchecaria, N. (2019). Konsentrasi hambat minimum (khm) kadar ekstrak etanol batang bajakah tampala (spatholobus littoralis hassk) terhadap bakteri escherichia coli melalui metode sumuran. Jurnal Ilmiah Manuntung, 5(2), Article 2. https://doi.org/10.51352/jim.v5i2.267
  23. Sinung, P., Nugroho, A., & Syaifudin, A. (2019). Pengaruh ph terhadap kadar flavonoid pada gel ekstrak daun kitolod (Hipobroma longiflora (L) G.Don). Farmasindo Politeknik Indonesia Surakarta, 3(1).
  24. Trinovani, E., Kusmiyati, M., Sudaryat, Y., & Rhamadianto, M. I. (2022). Penetapan kadar antosianin total dan aktivitas antioksidan ekstrak air, metanol, etanol 70% tape ketan hitam: determination of anthocyanin and antioxidant activities of water, methanol, ethanol 70% extract black glutinous rice. Medical Sains : Jurnal Ilmiah Kefarmasian, 7(4), 983–992. https://doi.org/10.37874/ms.v7i4.553